Which of the following processes does *not* directly contribute to the build-up of the proton gradient across the thylakoid membrane during non-cyclic photophosphorylation?

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Appears in NEET UG
Solution ✔ Verified
  • ASplitting of water molecules on the lumen side.
  • BPumping of protons from the stroma to the lumen by the cytochrome b6f complex.
  • CReduction of NADP+ on the stroma side.
  • DMovement of electrons through Photosystem I.
Explanation

The proton gradient is established by: 1) Water splitting (releases protons into the lumen). 2) Proton pumping by the cytochrome b6f complex (from stroma to lumen). 3) Consumption of protons from the stroma when NADP+ is reduced to NADPH (effectively increasing H+ concentration in the lumen relative to stroma). The movement of electrons *through Photosystem I itself* does not directly add protons to the lumen or remove them from the stroma to build the gradient, though it is part of the overall electron flow.

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